Journal: bioRxiv

Article Title: Dual medial prefrontal cortex and hippocampus projecting neurons in the paraventricular nucleus of the thalamus

doi: 10.1101/2021.10.02.462854

Figure Lengend Snippet: A) Paired bilateral injections of retrograde AAV-CAG-tdTomato (magenta) and retrograde AAV-CAG-GFP (green) delivered in mPFC and vHC for florescence protein expression of neurons in PVT. B) mPFC injection spread was confined to PL/IL regions, primarily layers V/VI (n = 4). Epifluorescence capture (4X) on the left depict the injection spread in PL/IL in one case (NCL 235). Retrograde labeling can be seen locally and in other frontal regions (VO, LO, ACC). On the right, a schematic representation of injection spread for each case across brain levels are illustrated. C) vHC injection spread was confined to CA1 molecular layers (n = 4). Epifluorescence capture (4X) on the left depict the injection spread in vCA1 in one case (NCL 235). Retrograde labeling can be seen locally in CA1, Sub and EC. On the right, a schematic representation of injection spread in vCA1 for each case across brain levels are illustrated. Abbreviations: AAV, adeno-associated virus; ACC, anterior cingulate cortex; CA1, CA1 subfield of hippocampus; CAG, chicken beta-Actin promoter; DG, dentate gryus; GFP, green fluorescent protein; HC, hippocampus; IL, infralimbic cortex; LO, lateral orbital cortex; mPFC, medial prefrontal cortex; PL, prelimbic cortex; rad, stratum radiatum; slm, stratum lacunosum moleculare; tdTomato, tandem dimer Tomato; vCA1, ventral CA1; VO, ventral orbital cortex.

Article Snippet: Retrograde AAV vectors AAV-CAG-tdTomato (59462-AAVrg; Addgene, MA) and AAV-CAG-GFP (Addgene, MA; 37825-AAVrg) were delivered bilaterally in mPFC (AP +3.0 mm, ML +/-1.2mm, DV -5.0mm, 50nL; -4.4mm, 150 nL; and -3.8mm, 200 nL) and vHC (AP -5.6mm, ML+/-5.85mm, DV -7.2 mm,100 nL; 6.8 mm, 200 nL, and 6.2 mm, 200 nL), respectively (see ).

Techniques: Expressing, Injection, Labeling, Virus